Temperature-sensitive mutants of the mycobacterium plasmid pAL5000


C. Guilhot, Unité de Génie Microbiologique (URA1300 du CNRS) Institut Pasteur, F-75015 Paris, France.


Abstract Two plasmids were isolated as thermosensitive replicons following in vitro mutagenesis of pB4, a pAL5000 derivative mycobacteria/Escherichia coli shuttle plasmid. Plasmids pCG59 and pCG63 replicate at 30°C but not at 39°C. This will allow their utilisation for transposon delivery, site-specific integration, or allele exchange.