Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate

  1. Martine Malissard1,2,
  2. Colette Duez2,
  3. Micheline Guinand1,
  4. Marie-Jeanne Vacheron1,
  5. Georges Michel1,
  6. Nicole Marty3,
  7. Bernard Joris2,
  8. Iris Thamm2,
  9. Jean-Marie Ghuysen2,*

Article first published online: 17 JAN 2006

DOI: 10.1111/j.1574-6968.1993.tb06302.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 110, Issue 1, pages 101–106, June 1993

Additional Information

How to Cite

Malissard, M., Duez, C., Guinand, M., Vacheron, M.-J., Michel, G., Marty, N., Joris, B., Thamm, I. and Ghuysen, J.-M. (1993), Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate. FEMS Microbiology Letters, 110: 101–106. doi: 10.1111/j.1574-6968.1993.tb06302.x

Author Information

  1. 1

    Laboratoire de Biochimie Microbienne, Université Claude Bernard, Villeurbanne, France

  2. 2

    Center d'Ingénierie des Protéines, Université de Liège, Sart Tilman, Belgium

  3. 3

    Laboratoire de Microbiologie, Université Paul Sabatier, Toulouse, France

* J.M. Ghuysen, CIP, Université de Liège, Institut de Chimie, B6, B-4000 Sart Tilman (Liège 1), Belgium.

Publication History

  1. Issue published online: 17 JAN 2006
  2. Article first published online: 17 JAN 2006
  3. (Received 22 March 1993, Accepted 23 March 1993)

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Keywords:

  • (poly ManA) Alginate lyase;
  • Gene cloning;
  • Primary structure;
  • Pseudomonas aeruginosa

Abstract The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originated from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870–1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265-amino acid residue alginate lyase showed lytic activity on a Pseudomonas aeruginosa alginate isolated from a cystic fibrosis patient. Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385–8391).

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