Isoprenoid biosynthesis in bacteria: Two different pathways?
Article first published online: 17 JAN 2006
FEMS Microbiology Letters
Volume 111, Issue 2-3, pages 135–140, August 1993
How to Cite
Horbach, S., Sahm, H. and Welle, R. (1993), Isoprenoid biosynthesis in bacteria: Two different pathways?. FEMS Microbiology Letters, 111: 135–140. doi: 10.1111/j.1574-6968.1993.tb06375.x
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- (Received 30 March 1993, Revised 28 April 1993, Accepted 29 April 1993)
- Isoprenoid pathway;
- Mevalonic acid;
Abstract The biosynthesis of isopentenylpyrophosphate, a central intermediate of isoprenoid formation, was investigated in six different bacterial organisms. Cell-free extracts of Myxococcus fulvus, Staphylococcus carnosus, Lactobacillus plantarum and Halobacterium cutirubrum converted [14C]acetyl-CoA or [14C]hydroxymethylglutaryl-CoA to [14C]mevalonic acid. Furthermore, [14C]mevalonic acid, [14C]mevalonate-5-phosphate and [14C]mevalonate-5-pyrophosphate were metabolized to [14C]isopentenylpyrophosphate in bacteria. In contrast, no intermediates of this reaction sequence could be detected using cell-free extracts of Zymomonas mobilis and Escherichia coli. These results indicate that at least two different pathways for the biosynthesis of isopentenylpyrophosphate are present in bacteria.