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Keywords:

  • Luciferase;
  • Campylobacter;
  • Promoter probe;
  • Flagellin;
  • Reporter gene

Abstract A novel promoter probe plasmid (pSP73) was constructed to allow the analysis of environmentally regulated gene expression in Campylobacter. The vector utilizes the luxAB genes from Xenorhabdus luminescens, which encode a thermostable luciferase, as reporters of gene expression. The utility of this reporter system was demonstrated by placing the expression of luxAB under the transcriptional control of the flaA gene promoter.