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Keywords:

  • Polymerase chain reaction;
  • Restriction site polymorphism;
  • Ribosomal RNA gene;
  • Borrelia;
  • Lyme disease

Abstract Three phyletic groups of Borrelia associated with Lyme disease, B. burgdorferi, B. garinii and group VS461 can be distinguished from each other and other species of Borrelia by BfaI restriction site polymorphisms in PCR amplified 16S rRNA genes. One strain isolated from an Ixodes pacificus tick in California that was previously unclassifiable was distinguishable from B. burgdorferi by an MnlI restriction site polymorphism.