Species of Borrelia distinguished by restriction site polymorphisms in 16S rRNA genes


Correspondence to: Michael McClelland, California Institute of Biological Research, 11099 North Torrey Pines Road, La Jolla, CA 92037, USA.


Abstract Three phyletic groups of Borrelia associated with Lyme disease, B. burgdorferi, B. garinii and group VS461 can be distinguished from each other and other species of Borrelia by BfaI restriction site polymorphisms in PCR amplified 16S rRNA genes. One strain isolated from an Ixodes pacificus tick in California that was previously unclassifiable was distinguishable from B. burgdorferi by an MnlI restriction site polymorphism.