Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampCβ-lactamase promoter

Authors


Correspondence to: S. Busby, School of Biochemistry, University of Birmingham, Birmingham B15 2TT, UK.

Abstract

Abstract The complete sequence of the Pseudomonas aeruginosaβ-lactamase regulator gene ampR has been determined. The amino acid sequence encoded by this gene is compared with other members of the LysR family of transcription factors. The P. aeruginosa ampC promoter was subcloned and gel mobility shift assays were used to demonstrate specific binding of AmpR to the ampC promoter region.

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