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Evaluation of macrolide and lincosamide antibiotics for plasmid maintenance in low pH Clostridium acetobutylicum ATCC 824 fermentations
Article first published online: 17 JAN 2006
DOI: 10.1111/j.1574-6968.1993.tb06490.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
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How to Cite
Mermelstein, L. D. and Papoutsakis, E. T. (1993), Evaluation of macrolide and lincosamide antibiotics for plasmid maintenance in low pH Clostridium acetobutylicum ATCC 824 fermentations. FEMS Microbiology Letters, 113: 71–75. doi: 10.1111/j.1574-6968.1993.tb06490.x
Publication History
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- (Received 6 July 1993, Accepted 16 July 1993)
- Abstract
- References
- Cited By
Keywords:
- Clostridium acetobutylicum;
- Macrolide and lincosamide instability;
- Plasmid maintenance;
- Recombinant fermentation;
- Low pH fermentation
Abstract Clarithromycin, midecamycin and tylosin effectively provided selective pressure for maintenance of a macrolide, lincosamide and streptogramin B resistance (MLSr) plasmid in Clostridium acetobutylicum ATCC 824 at pH values as low as 4.5, whereas erythromycin, oleandomycin, spiramycin, lincomycin and clindamycin did not. Furthermore, clarithromycin, midecamycin and tylosin were not degraded by late stationary-phase ATCC 824 fermentation fluids. Solvent formation, but not acid formation. was inhibited by tylosin but not by clarithromycin in an MLSr plasmid containing strain. Clarithromycin should therefore be useful for the maintenance of selective pressure for MLSr plasmids in low pH recombinant C. acetobutylicum ATCC 824 fermentations.