Identification and sequencing of pyrG, the CTP synthetase gene of Azospirillum brasilense Sp7

  1. Wolfgang Zimmer*,
  2. Barbara Hundeshagen

Article first published online: 17 JAN 2006

DOI: 10.1111/j.1574-6968.1994.tb06650.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 115, Issue 2-3, pages 273–277, January 1994

Additional Information

How to Cite

Zimmer, W. and Hundeshagen, B. (1994), Identification and sequencing of pyrG, the CTP synthetase gene of Azospirillum brasilense Sp7. FEMS Microbiology Letters, 115: 273–277. doi: 10.1111/j.1574-6968.1994.tb06650.x

Author Information

  1. Botanisches Institut der Universität zu Köln, Gyrhofstraβe 15, D-50923 Köln, FRG

* *Corresponding author. Tel.: (0221) 470 2480; Fax: (0221) 470 5181

Publication History

  1. Issue published online: 17 JAN 2006
  2. Article first published online: 17 JAN 2006
  3. (Received 19 October 1993, Revised 5 November 1993, Accepted 8 November 1993)

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Keywords:

  • CTP synthetase gene;
  • pyrG;
  • Dienelactone hydrolase gene homologue;
  • Azospirillum brasilense

Abstract An 18.5-kb DNA fragment carrying the trpGDC cluster of Azospirillum brasilense Sp7 was previously cloned, yielding cosmid pAB1005. Attempts to identify trpA in the vicinity of trpGDC failed but led to the detection of a locus strongly homologous to pyrG, the structural gene for the CTP synthetase. The function of the A. brasilense pyrG gene was verified by complementation of the cytidine-requiring PyrG-deficient mutant JF646 of Escherichia coli. A second open reading frame was identified downstream of pyrG. The deduced amino acid sequence showed homology to dienelactone hydrolases of Pseudomonas and Alcaligenes, enzymes involved in utilization of halogenated aromatic compounds.

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