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Anaerobic respiration of Shewanella putrefaciens requires both chromosomal and plasmid-borne genes
Article first published online: 17 JAN 2006
DOI: 10.1111/j.1574-6968.1994.tb06900.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
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How to Cite
Saffarini, D.A., DiChristina, T.J., Bermudes, D. and Nealson, K.H. (1994), Anaerobic respiration of Shewanella putrefaciens requires both chromosomal and plasmid-borne genes. FEMS Microbiology Letters, 119: 271–277. doi: 10.1111/j.1574-6968.1994.tb06900.x
Publication History
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- (Received 28 February 1994, Revised 5 April 1994, Accepted 6 April 1994)
- Abstract
- References
- Cited By
Keywords:
- Anaerobic respiration;
- Shewanella putrefaciens;
- Respiratory mutant;
- Iron reduction;
- Manganese reduction
Abstract Pleiotropic respiratory mutants, incapable of growth on any electron acceptor other than oxygen, were isolated from two strains of Shewanella putrefaciens (MR-1 and sp200). All anaerobic respiratory functions were restored by complementation of the mutants with specific cloned DNA fragments. Southern hybridization experiments revealed that the fragment that complements the MR-1 mutant was localized on the megaplasmids of both strains, while the fragment that complements the sp200 mutant was chromosomal. Neither of these fragments hybridized with the anaerobic regulatory genes of S. putrefaciens (etrA) or E. coli (fnr).