Cloning and DNA sequence analysis of bmpC, a gene encoding a potential membrane lipoprotein of Borrelia burgdorferi

  1. Lieselotte Aron1,
  2. Michael Alekshun2,
  3. Lorah Perlee2,
  4. Ira Schwartz2,
  5. Henry P. Godfrey3,
  6. Felipe C. Cabello1,*

Article first published online: 17 JAN 2006

DOI: 10.1111/j.1574-6968.1994.tb07204.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 123, Issue 1-2, pages 75–82, October 1994

Additional Information

How to Cite

Aron, L., Alekshun, M., Perlee, L., Schwartz, I., Godfrey, H. P. and Cabello, F. C. (1994), Cloning and DNA sequence analysis of bmpC, a gene encoding a potential membrane lipoprotein of Borrelia burgdorferi. FEMS Microbiology Letters, 123: 75–82. doi: 10.1111/j.1574-6968.1994.tb07204.x

Author Information

  1. 1

    Department of Microbiology and Immunology, New York Medical College, Valhalla, New York 10595, USA

  2. 2

    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA

  3. 3

    Department of Experimental Pathology, New York Medical College, Valhalla, New York 10595, USA

* *Corresponding author. Tel: (914) 993-4182; Fax: (914) 993-4176.

Publication History

  1. Issue published online: 17 JAN 2006
  2. Article first published online: 17 JAN 2006
  3. (Received 25 July 1994, Accepted 16 August 1994)

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Keywords:

  • Borrelia burgdorferi;
  • TmpC gene;
  • Lyme disease

Abstract Immunoscreening of a λgt11 genomic library of Borrelia burgdorferi expressed in Escherichia coli permitted detection of a clone containing a partial sequence of a B. burgdorferi gene encoding a protein with significant homology to TmpC of Treponema pallidum. Subsequent cloning and DNA sequence analysis revealed an open reading frame encoding a protein with 353 amino acid residues. The open reading frame is preceded by putative promoter sequences and a ribosome binding site, and is initiated with a TTG. The putative protein shares 26% identity with TmpC, contains a signal peptidase II sequence, and is also homologous to the gene products of the recently described bmpA and bmpB of B. burgdorferi. This gene has been designated bmpC. Additional sequencing and restriction analysis indicate that it is located at approximately 400 kbp on the chromosomal map of B. burgdorferi, immediately upstream of bmpA and bmpB.

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