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Host-plasmid interactions in recombinant strains of Clostridium acetobutylicum ATCC 824
Article first published online: 17 JAN 2006
DOI: 10.1111/j.1574-6968.1994.tb07245.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
Additional Information
How to Cite
Walter, K. A., Mermelstein, L. D. and Papoutsakis, E. T. (1994), Host-plasmid interactions in recombinant strains of Clostridium acetobutylicum ATCC 824. FEMS Microbiology Letters, 123: 335–341. doi: 10.1111/j.1574-6968.1994.tb07245.x
Publication History
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- (Received 18 May 1993, Revised 17 August 1994, Accepted 7 September 1994)
- Abstract
- References
- Cited By
Keywords:
- Clostridium acetobutylicum;
- Solvent production;
- Recombinat fermentation;
- Host-plasmid interaction
Abstract Plasmid-containing strains of Clostridium acetobutylicum produced higher levels of solvents and lower levels of acids than wild-type cells in controlled pH 4.5 batch fermentations. This effect was observed regardless of whether or not the plasmids contained C. acetobutylicum genes. The effect was less prevalent in higher pH fermentations and apparently independent of the actual DNA sequences contained on these plasmids. The plasmid-containing strains were found to have lower growth-rates and higher solventogenic enzyme activities than wild-type cells. However, similar activity levels were found for both butyrate-pathway enzymes.