1574-6968/asset/FML_left.gif?v=1&s=d9ad90a5f75253894fe5059aa2f75bf910ebf83a)
1574-6968/asset/FML_right.gif?v=1&s=48d5e33deef512c09651020f71074ad93d3351e7)
Genes encoding homologues of three consecutive enzymes in the butyrate/butanol-producing pathway of Clostridium acetobutylicum are clustered on the Clostridium difficile chromosome
Article first published online: 17 JAN 2006
DOI: 10.1111/j.1574-6968.1994.tb07262.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
Additional Information
How to Cite
Mullany, P., Clayton, C. L., Pallen, M. J., Slone, R., Al-Saleh, A. and Tabaqchali, S. (1994), Genes encoding homologues of three consecutive enzymes in the butyrate/butanol-producing pathway of Clostridium acetobutylicum are clustered on the Clostridium difficile chromosome. FEMS Microbiology Letters, 124: 61–67. doi: 10.1111/j.1574-6968.1994.tb07262.x
Publication History
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- (Received 8 August 1994, Revised 16 September 1994, Accepted 21 September 1994)
- Abstract
- References
- Cited By
Keywords:
- Clostridium difficile;
- Butyrate/butanol production;
- Fermentation operon
Abstract Screening of a Clostridium difficileψEMBL3 gene library with antisera raised against C. difficile culture supernatant identified several clones expressing a 31-kDa protein. A 1.8-kb HindIII fragment subcloned from one of the clones was sufficient for expression of the 31-kDa polypeptide. Southern blot analysis showed a region homologous to this fragment to be present in all of 13 different C. difficile strains tested. Sequence analysis of the 1.8-kb fragment revealed three adjacent open reading frames. A database search showed that these three open reading frames appeared to encode homologues of three consecutive enzymes in the butanol/butyrate-producing pathway of Clostridium acetobutylicum (crotonase, β-hydroxybutyryl coenzyme A dehydrogenase and thiolase).