Variability of peptidoglycan structural parameters in Gram-negative bacteria

Authors

  • JoséCarlos Quintela,

    1. Centra de B iología Molecular ‘Severo Ochoa’ C.S.I.C., -UA.M., Facultad de Ciencias (Biología), U.A.M., Campus de Cantoblanco, 28049 Madrid, Spain
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  • Marta Caparrós,

    1. Centra de B iología Molecular ‘Severo Ochoa’ C.S.I.C., -UA.M., Facultad de Ciencias (Biología), U.A.M., Campus de Cantoblanco, 28049 Madrid, Spain
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  • Miguel A. de Pedro

    Corresponding author
    1. Centra de B iología Molecular ‘Severo Ochoa’ C.S.I.C., -UA.M., Facultad de Ciencias (Biología), U.A.M., Campus de Cantoblanco, 28049 Madrid, Spain
      *Corresponding author. Tel: +34 (1) 397-8083; Fax: +34 (1) 397-8344.
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*Corresponding author. Tel: +34 (1) 397-8083; Fax: +34 (1) 397-8344.

Abstract

Abstract Muropeptide composition of peptidoglycan from the Gram-negative bacteria Aeromonas sp., Acinetobacter acetoaceticus, Agrobacterium tumefaciens, Enterobacter cloacae, Proteus morganii, Pseudomonas aeruginosa, Pseudomonas putida, Vibrio parahaemolyticus Yersinia enterocolitica and Escherichia coli, was analyzed by HPLC In all instances peptidoglycan was built up from the same subunits. A wide disparity in the relative abundance of muropeptides and all structural parameters was observed. The contribution of LD-A2pm-A2pm cross-linked muropeptides was extremely variable; from 1 to 45% of cross-linked muropeptides. Muropeptides with the dipeptides Lys-Lys or Arg-Lys, indicative of murein-bound (lipo)proteins, were detected in all instances although abundance was very variable.

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