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Functional cloning of the dihydropteroate synthase gene of Staphylococcus haemolyticus
Article first published online: 17 JAN 2006
DOI: 10.1111/j.1574-6968.1995.tb07932.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
Additional Information
How to Cite
Kellam, P., Dallas, W. S., Ballantine, S. P. and Delves, C. J. (1995), Functional cloning of the dihydropteroate synthase gene of Staphylococcus haemolyticus. FEMS Microbiology Letters, 134: 165–169. doi: 10.1111/j.1574-6968.1995.tb07932.x
Publication History
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- (Received 6 October 1995, Accepted 6 October 1995)
- Abstract
- References
- Cited By
Keywords:
- Staphylococcus aureus;
- Dihydropteroate synthase;
- Folate biosynthesis;
- Functional gene cloning;
- Sequence analysis
Abstract A 1.7-kilobase fragment of the Staphylococcus haemolyticus chromosome containing the dihydropteroate synthase gene has been cloned by complementation in a temperature-sensitive mutant of Escherichia coli. The gene, designated folP, predicts a gene product of 29613 Da which shares significant amino acid sequence identity with other known bacterial dihydropteroate synthases. Analysis of the DNA sequence upstream and downstream of folP identified two further, incomplete open reading frames, one of which shows predicted amino acid sequence similarity to a second bacterial folic acid synthesis enzyme, dihydroneopterin aldolase.