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A novel method for the isolation of mycobacterial DNA
Article first published online: 17 JAN 2006
FEMS Microbiology Letters
Volume 135, Issue 1, pages 71–77, January 1996
How to Cite
Gonzalez-y-Merchand, J. A., Estrada-Garcia, I., Colston, M. J. and Cox, R. A. (1996), A novel method for the isolation of mycobacterial DNA. FEMS Microbiology Letters, 135: 71–77. doi: 10.1111/j.1574-6968.1996.tb07968.x
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- (Received 28 August 1995, Revised 25 October 1995, Accepted 27 October 1995)
- DNA isolation;
- rRNA operon
Abstract DNA was isolated from mycobacteria by a simplified procedure. Cells were suspended in 6 M guanidinium chloride, the suspension was cooled to −70 °C, then incubated at 65 °C for 10 min, cooled in ice, deproteinized by chloroform and DNA was recovered from the supernatant. The procedure was used to obtain DNA from several mycobacteria (1 × 109) or more cells) including Mycobacterium neoaurum M. fortuitum M. phlei and M. smegmatis. Each of the species was shown to have two ribosomal RNA operons per genome, and preliminary evidence was obtained which suggests that one of these operons is homologous with one of the operons of M. smegmatis.