Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae

Authors

  • Joakim Norbeck,

    1. Department of General and Marine Microbiology, Lundberg Laboratory, University of Göteborg, Medicinaregatan 9C, 413 90 Göteborg, Sweden
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  • Anders Blomberg

    Corresponding author
    1. Department of General and Marine Microbiology, Lundberg Laboratory, University of Göteborg, Medicinaregatan 9C, 413 90 Göteborg, Sweden
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*Corresponding author. Tel: +46 (31) 773 2589; fax: + 46 (31) 773 2599; E-mail: anders.blomberg@gmm.gu.se

Abstract

AbstractSaccharomyces cerevisiae exponentially growing in basic or 0.7 M NaCl medium were isotopically labelled with 35S-methionine, followed by protein separation and quantification by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) combined with computerised image analysis. The electrophoretic separation resolved about 650 proteins of which 13 displayed significant and at least 2-fold changes in rate of synthesis during saline growth. By sequencing of 2D-PAGE resolved proteins, one of the 8 induced spot, p42.9/5.5, was shown to correspond to the full length (containing the N-terminal extension) product of the GPD1 gene encoding the cytoplasmic glycerol 3-phosphate dehydrogenase. The expression of the TDH3 gene, glyceraldehyde 3-phosphate dehydrogenase, and the ENO2 gene, enolase, decreased during growth in NaCl medium, declines hypothesised to have an impact on the flux to glycerol.

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