Genomic organization of purK and purE in Brevibacterium ammoniagenes ATCC 6872: purE locus provides a clue for genomic evolution

  1. Sung-Oh Chung1,3,
  2. Jin-Ho Lee1,
  3. Se Yong Lee2,
  4. Dae-Sil Lee3,*

Article first published online: 17 JAN 2006

DOI: 10.1111/j.1574-6968.1996.tb08116.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 137, Issue 2-3, pages 265–268, April 1996

Additional Information

How to Cite

Chung, S.-O., Lee, J.-H., Lee, S. Y. and Lee, D.-S. (1996), Genomic organization of purK and purE in Brevibacterium ammoniagenes ATCC 6872: purE locus provides a clue for genomic evolution. FEMS Microbiology Letters, 137: 265–268. doi: 10.1111/j.1574-6968.1996.tb08116.x

Author Information

  1. 1

    Research and Development Center, Cheil Foods and Chemicals Inc. 522-1, Dokpyong-ri, Majang-myon, Ichon-kun, Kyonggi-do, South Korea

  2. 2

    Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Korea University, Seoul, South Korea

  3. 3

    Molecular Biology Laboratory, Korea Research Institute of Bioscience and Biotechnology, KIST, Taejon, 305-606, South Korea

* *Corresponding author. Tel.: +82 (42) 860 4110; Fax: +82 (42) 860 4593; E-mail: daesilee@geri4680.geri.re.kr

Publication History

  1. Issue published online: 17 JAN 2006
  2. Article first published online: 17 JAN 2006
  3. (Received 12 December 1995, Revised 8 February 1996, Accepted 10 February 1996)

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (362K)

Keywords:

  • Brevibacterium ammoniagenes;
  • purk;
  • pure;
  • Genomic organization

Abstract From the genomic library of Brevibacterium ammoniagenes ATCC6872, the purE locus encoding 5′-phosphoribosyl-5aminoimidazole (AIR) carboxylase (EC 4.1.1.21) was cloned and its nucleotide sequence was determined. From the sequence analysis, two distinct open reading frames (ORFs) in the sequence of the purE locus were identified as purK and purE genes (purK-purE). An in vivo translation experiment reconfirmed the purK and purE genes to be independent. The genomic organization in the purE locus of B. ammoniagenes is opposite to that of the bacteria Escherichia coli and Bacillus subtilis. However, it coincides with the fused genes (purKE) of higher organisms Saccharomyces cerevisiae, Schizosaccharomyces pombe and Vigna aconitifolia. This suggests that the purE locus might be an intermediate form for genomic evolution of bacteria to higher organisms.

Get PDF (362K)