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Cloning, nucleotide sequence and expression of the gene encoding the cellulose-binding protein 1 (CBP1) of Fibrobacter succinogenes S85
Article first published online: 17 JAN 2006
DOI: 10.1111/j.1574-6968.1996.tb08177.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
Additional Information
How to Cite
Mitsumori, M., Minato, H., Sekizaki, T., Uchida, I. and Ito, H. (1996), Cloning, nucleotide sequence and expression of the gene encoding the cellulose-binding protein 1 (CBP1) of Fibrobacter succinogenes S85. FEMS Microbiology Letters, 139: 43–50. doi: 10.1111/j.1574-6968.1996.tb08177.x
Publication History
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- (Received 14 February 1996, Revised 18 March 1996, Accepted 20 March 1996)
- Abstract
- References
- Cited By
Keywords:
- Fibrobacter succinogenes;
- Cellulose binding;
- Fused protein;
- Avicel
Abstract The nucleotide sequence of the gene encoding the Fibrobacter succinogenes S85 cellulose-binding protein 1 (CBP1) has been determined. The gene encodes a protein of 1054 amino acids with a molecular mass of 118614. The deduced amino acid sequence of CBPl showed an extensive similarity to the cellulose-binding domain of an endoglucanase (EGCCD) from Clostridium cellulolyticum and contained the reiterated regions. The cloned gene was inserted into an expression vector, pRSETA, and was expressed in E. coli as a fused protein with the peptide consisting of six consecutive histidine residues. The fused protein was detected by immunoblotting using antiserum against CBP1, and exhibited the cellulose-binding activities.