Identification and characterization of the pqqDGC gene cluster involved in pyrroloquinoline quinone production in an obligate methylotroph Methylobacillus flagellatum

  1. Mark Gomelsky1,2,*,
  2. Francis Biville1,2,
  3. Francis Gasser2,
  4. Yuri D. Tsygankov1

Article first published online: 17 JAN 2006

DOI: 10.1111/j.1574-6968.1996.tb08380.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 141, Issue 2-3, pages 169–176, August 1996

Additional Information

How to Cite

Gomelsky, M., Biville, F., Gasser, F. and Tsygankov, Y. D. (1996), Identification and characterization of the pqqDGC gene cluster involved in pyrroloquinoline quinone production in an obligate methylotroph Methylobacillus flagellatum. FEMS Microbiology Letters, 141: 169–176. doi: 10.1111/j.1574-6968.1996.tb08380.x

Author Information

  1. 1

    Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia

  2. 2

    Département de Biochimie et Génétique Moléculaire, Institut Pasteur, Paris, France

* *Corresponding author. Present address: Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA. Tel: + 1 (713) 794 1744 ext. 1524; Fax: +1 (713) 794 1782; E-mail: gomelsky@utmmg.med.uth.tmc.edu

Publication History

  1. Issue published online: 17 JAN 2006
  2. Article first published online: 17 JAN 2006
  3. (Received 18 March 1996, Revised 14 May 1996, Accepted 20 May 1996)

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Keywords:

  • Methylotroph;
  • Methylobacillus flagellatum;
  • Pyrroloquinoline quinone;
  • pqq Genes;
  • PqqG;
  • Proteobacteria

Abstract Pyrroloquinoline quinone is a prosthetic group of bacterial methanol dehydrogenases as well as some alcohol and glucose dehydrogenases. Genes involved in pyrroloquinoline quinone production have previously been cloned from the representatives of the α and γ subdivisions of the Proteobacteria. We report identification and the sequence of the pqqDGC gene cluster in the obligate methylotroph, Methylobacillus flagellatum, which belongs to the β subdivision. The deduced products of the pqq genes from M. flagellatum appear to be more similar to their counterparts from non-methylotrophic species of the γ subdivision than to a facultative methylotroph of the a subdivision. A non-polar mutation in pqqG was constructed and resulted in a strain impaired in growth on methanol. This mutant accumulated a detectable amount of intracellular pyrroloquinoline quinone, but in contrast to the wild type, did not excrete pyrroloquinoline quinone into the culture medium. The possible role of PqqG is discussed.

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