A general approach for cloning and characterizing dNDP-glucose dehydratase genes from actinomycetes

  1. Heinrich Decker1,†,
  2. Sibylle Gaisser2,
  3. Stefan Pelzer1,
  4. Peter Schneider1,
  5. Lucia Westrich2,
  6. Wolfgang Wohlleben1,
  7. Andreas Bechthold2,*

Article first published online: 17 JAN 2006

DOI: 10.1111/j.1574-6968.1996.tb08384.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 141, Issue 2-3, pages 195–201, August 1996

Additional Information

How to Cite

Decker, H., Gaisser, S., Pelzer, S., Schneider, P., Westrich, L., Wohlleben, W. and Bechthold, A. (1996), A general approach for cloning and characterizing dNDP-glucose dehydratase genes from actinomycetes. FEMS Microbiology Letters, 141: 195–201. doi: 10.1111/j.1574-6968.1996.tb08384.x

Author Information

  1. 1

    Eberhard-Karls-Universität Tübingen, Mikrobiologie, D-72076 Tübingen, Germany

  2. 2

    Eberhard-Karts Universität Tübingen, Pharmazeutisches Institut, Pharmazeutische Biologie, D-72076 Tübingen, Germany

  1. †

    Hoechst AG, Biologica H780, D-65926 Frankfurt, Germany.

* *Corresponding author.

Publication History

  1. Issue published online: 17 JAN 2006
  2. Article first published online: 17 JAN 2006
  3. (Received 15 May 1996, Revised 29 May 1996, Accepted 30 May 1996)

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Keywords:

  • Actinomyces;
  • Streptomyces;
  • Polymerase chain reaction;
  • 6-Deoxyhexose;
  • Genetic screening of dNDP-glucose dehydratases

Abstract Oligonucleotide primers were designed and successfully applied to amplify DNA fragments of dNDP-glucose dehydratase genes from actinomycete species producing natural compounds which contain deoxysugar moieties. The deduced amino acid sequence of the isolated fragments revealed similarity to known dNDP-glucose dehydratases. A phylogeny for the deduced proteins of the obtained fragments and for dNDP-glucose dehydratases described in the data bases was constructed. dNDP-glucose dehydratases from actinomycetes were more related to each other than to dehydratases from species of other orders. The phylogenetic analysis also revealed a close relation between dehydratases from strains producing natural compounds with similar deoxysugar moieties.

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