A collagen binding protein from Lactobacillus reuteri is part of an ABC transporter system?
Article first published online: 17 JAN 2006
FEMS Microbiology Letters
Volume 144, Issue 1, pages 33–38, October 1996
How to Cite
Roos, S., Aleljung, P., Robert, N., Lee, B., Wadström, T., Lindberg, M. and Jonsson, H. (1996), A collagen binding protein from Lactobacillus reuteri is part of an ABC transporter system?. FEMS Microbiology Letters, 144: 33–38. doi: 10.1111/j.1574-6968.1996.tb08505.x
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- (Received 10 July 1996, Revised 12 August 1996, Accepted 14 August 1996)
- Lactobacillus reuteri;
- ABC transporter
Abstract The gene coding for a collagen binding protein from Lactobacillus reuteri NCIB 11951 was cloned and sequenced. A genomic lambda library was constructed and recombinant plaques were screened using antisera raised against purified collagen binding proteins from the same L. reuteri strain. The positive plaques were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis, which revealed the expression of a 29 kDa protein, which reacted with the antisera and bound 125I-labelled type I collagen. The sequence of the corresponding gene, cnb showed that the collagen binding protein has sequence similarities to the solute binding component of bacterial ABC transporters.