Methanoplanus petrolearius sp. nov., a novel methanogenic bacterium from an oil-producing well

Authors

  • B Ollivier,

    1. Laboratoire ORSTOM de Microbiologie des Anaérobies, Université de Provence, CESB-ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Search for more papers by this author
  • J.-L Cayol,

    1. Laboratoire ORSTOM de Microbiologie des Anaérobies, Université de Provence, CESB-ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Search for more papers by this author
  • B.K.C Patel,

    Corresponding author
    1. School of Biomolecular and Biomedical Sciences, Faculty of Science and Technology, Griffith University, Brisbane, Queensland 4111, Australia
    Search for more papers by this author
  • M Magot,

    1. Sanofi Recherche, Groupe Elf-Aquitaine, Unité de Microbiologie, 31676 Labège Cedex, France
    Search for more papers by this author
  • M.-L Fardeau,

    1. Laboratoire ORSTOM de Microbiologie des Anaérobies, Université de Provence, CESB-ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Search for more papers by this author
  • J.-L Garcia

    1. Laboratoire ORSTOM de Microbiologie des Anaérobies, Université de Provence, CESB-ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Search for more papers by this author

Corresponding author. Tel.: +61 (7) 3875 7695; fax: +61 (7) 3875 7656; e-mail: b.patel@sct.gu.edu.au

Abstract

A disc-shaped methanogenic bacterium designated strain SEBR 4847T (T=type strain) was isolated from a sample collected from an African offshore oil field. Strain SEBR 4847T was non-motile, had a G+C content of 50 mol% and produced methane from H2+CO2, formate, and CO2+propanol. Strain SEBR 4847T grew optimally at 37°C; no growth was observed at 25°C or 45°C. It grew in the presence of up to 50 g/l NaCl; 10–30 g/l was required for optimal growth. The optimum pH for growth was 7.0. Doubling time was about 10 h under optimal conditions. Based on 16S rRNA sequence analysis, the isolate was identified as a new species of the genus Methanoplanus and designated Methanoplanus petrolearius sp. nov. The type strain is SEBR 4847T (=OCM 486).

Ancillary