Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2BB, UK.
Transcriptional analysis of the sigA and sigB genes of Brevibacterium lactofermentum
Article first published online: 17 JAN 2006
FEMS Microbiology Letters
Volume 153, Issue 1, pages 111–117, August 1997
How to Cite
Oguiza, J. A., Marcos, A. T. and Martín, J. F. (1997), Transcriptional analysis of the sigA and sigB genes of Brevibacterium lactofermentum. FEMS Microbiology Letters, 153: 111–117. doi: 10.1111/j.1574-6968.1997.tb10471.x
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- Received 17 February 1997, Revised 10 May 1997, Accepted 21 May 1997
- Sigma factor;
Transcription of the sigA and sigB genes of Brevibacterium lactofermentum, encoding the principal sigma factors of RNA polymerase, has been investigated by Northern blot and primer extension analysis. Northern hybridizations revealed that sigA is transcribed as a monocistronic mRNA of 1.7 kb and sigB gives two transcripts of 1.1 and 1.5 kb. Similar transcription patterns of sigA and sigB genes in nutrient-rich medium were observed; transcripts of both genes occurred simultaneously throughout the exponential growth phase and decayed clearly when the stationary phase was reached. Primer extension analysis of B. lactofermentum RNA showed that the sigA and sigB transcription initiation sites are located 17 bp and 24 bp upstream from the first nucleotide of the respective translation initiation codons. Alignment of the sigA and sigB promoter regions provided evidence for highly conserved sequences in both of them.