• 1
    Homann, T., Tag, C., Biebl, H., Deckwer, W.-D. and Schink, B. (1990) Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains. Appl. Microbiol. Biotechnol. 33, 121126.
  • 2
    Heyndrickx, M., De Vos, P., Vacanneyt, M. and De Ley, J. (1991) The fermentation of glycerol by Clostridium butyricum LMG 1212 t2 and 1213 t1 and Clostridium pasteurianum LMG 3285. Appl. Microbiol. Biotechnol. 34, 637642.
  • 3
    Dabrock, B., Bahl, H. and Gottschalk, G. (1992) Parameters effecting solvent production by Clostridium pasteurianum. Appl. Environ. Microbiol. 58, 12331239.
  • 4
    Sprenger, G.A., Hammer, B.A., Johnson, E.A. and Lin, E.C.C. (1989) Anaerobic growth of Escherichia coli on glycerol by importing genes of the dha regulon from Klebsiella pneumoniae. J. Gen. Microbiol. 135, 12551262.
  • 5
    Daniel, R. and Gottschalk, G. (1992) Growth temperature-dependent activity of glycerol dehydratase in Escherichia coli expressing the Citrobacter freundii dha regulon. FEMS Microbiol. Lett. 100, 281286.
  • 6
    Daniel, R., Stuertz, K. and Gottschalk, G. (1995) Biochemical and molecular characterization of the oxidative branch of glycerol utilization by Citrobacter freundii. J. Bacteriol. 177, 43924401.
  • 7
    Daniel, R., Boenigk, R. and Gottschalk, G. (1995) Purification of 1,3-propanediol dehydrogenase from Citrobacter freundii: cloning sequencing and overexpression of the corresponding gene in Escherichia coli. J. Bacteriol. 177, 21512156.
  • 8
    Seyfried, M., Daniel, R. and Gottschalk, G. (1996) Cloning, sequencing and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii. J. Bacteriol. 178, 57935796.
  • 9
    Abbad-Andaloussi, S., Dürr, C., Raval, G. and Petitdemange, H. (1996) Carbon and electron flow in Clostridium butyricum grown in chemostat culture on glycerol and on glucose. Microbiology 142, 11491158.
  • 10
    Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1987) Current Protocols in Molecular Biology. John Wiley and Sons, New York.
  • 11
    Kell, D.B., Peck, M.W., Rodger, G. and Morris, J.G. (1981) On the permeability to weak acids and bases of the cytoplasmic membrane of Clostridium pasteurianum. Biochem. Biophys. Res. Commun. 99, 8188.
  • 12
    Marmur, J. (1961) A procedure for the isolation of desoxyribonucleic acid from microorganisms. J. Mol. Biol. 3, 208218.
  • 13
    Sanger, F., Nicklen, S. and Coulson, A. R (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463–5467.
  • 14
    Ruch, F.E., Lengeler, J. and Lin, E.C.C. (1974) Regulation of glycerol catabolism in Klebsiella aerogenes. J. Bacteriol. 119, 5056.
  • 15
    Johnson, E.A., Burke, S.K., Forage, R.G. and Lin, E.C.C. (1984) Purification and properties of dihydroxyacetone kinase from Klebsiella pneumoniae. J. Bacteriol. 160, 5560.
  • 16
    Toraya, T., Kazutoshi, U., Fukui, S. and Hogenkamp, H.P.C. (1977) Studies on the mechanism of the adenosyl-cobalamin-dependent dioldehydratase reaction by the use of analogs of the coenzyme. J. Biol. Chem. 252, 963970.
  • 17
    Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248254.
  • 18
    Andersson, L.O., Borg, H. and Mikaelsson, M. (1972) Molecular weight estimation of proteins by electrophoresis in polyacrylamide gel of graded porosity. FEBS Lett. 20, 199202.
  • 19
    Boenigk, R. (1991) Optimierung der 1,3-Propandiolbildung mit Citrobacter freundii und Isolierung und Charakterisierung der 1,3-Propandiol-Dehydrogenase. Doctoral Thesis, Georg-August-Universität, Göttingen.
  • 20
    Reid, M.F. and Fewson, C.A. (1994) Molecular characterization of microbial alcohol dehydrogenases. Crit. Rev. Microbiol. 20, 1356.
  • 21
    Bairoch, A. (1991) PROSITE: a dictionary of sites and patterns in proteins. Nucleic Acids Res. 19 (Suppl.), 2241–2245.
  • 22
    Tse, P., Scopes, R.K., Wedd, A.G., Bakshi, E. and Murray, K.S. (1988) An iron-activated alcohol dehydrogenase: metal dissociation constants and magnetic and spectroscopic properties. J. Am. Chem. Soc. 110, 12951297.
  • 23
    Conway, T. and Ingram, L.O. (1989) Similarity of Escherichia coli propanediol oxidoreductase (fucO product) and an unusual alcohol dehydrogenase from Zymomonas mobilis and Saccharomyces cerevisiae. J. Bacteriol. 171, 37543759.
  • 24
    Kessler, D., Herth, W. and Knappe, J. (1992) Ultrastructure and pyruvate formate-lyase radical quenching property of the multienzymic AdhE protein of Escherichia coli. J. Biol. Chem. 267, 1807318079.
  • 25
    Stojiljkovic, I., Bäumler, A.J. and Heffron, F. (1995) Ethanolamine utilization in Salmonella typhimurium: nucleotide sequence, protein expression, and mutational analysis of the cchA cchB eutE eutJ eutG eutH gene cluster. J. Bacteriol. 177, 13571366.
  • 26
    Wiliamson, V.M. and Paquin, C.E. (1987) Homology of Saccharomyces cerevisiae ADH4 to an iron-activated alcohol dehydrogenase from Zymomonas mobilis. Mol. Gen. Genet. 209, 374381.
  • 27
    Wierenga, R.K., DeMaeyer, M.C.H. and Hol, W.G.J. (1985) Interaction of pyrophosphate moieties with α-helixes in dinucleotide binding proteins. Biochemistry 24, 13461357.
  • 28
    Conway, T., Sewell, G.W., Osman, Y.A. and Ingram, L.O. (1987) Cloning and sequencing of the alcohol dehydrogenase II gene from. J. Bacteriol. 169, 25912597.
  • 29
    Youngleson, J.S., Jones, W.A., Jones, D.T. and Woods, D.R. (1988) Molecular analysis and nucleotide sequence of the adh1 gene encoding an NADPH-dependent butanol dehydrogenase gene in the Gram-positive anaerobe Clostridium acetobutylicum. Gene 78, 355364.
  • 30
    Söhling, B. and Gottschalk, G. (1996) Molecular Analysis of the anaerobic succinate degradation pathway in Clostridium kluyveri. J. Bacteriol. 178, 871880.
  • 31
    Goodlove, P.E., Cunningham, P.R., Parker, J. and Clark, D.P. (1989) Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli. Gene 85, 209214.
  • 32
    Fischer, R.J., Helms, J. and Dürre, P. (1993) Cloning, sequencing and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis. J. Bacteriol. 175, 66596669.
  • 33
    De Vries, G.E., Arfman, N., Terpstra, P. and Dijkhuizen, L. (1992) Cloning, expression, and sequence analysis of the Bacillus methanolicus C1 methanol dehydrogenase gene. J. Bacteriol. 174, 5346–5353
  • 34
    Walter, K.A., Bennett, G.N. and Papoutsakis, E.T. (1992) Molecular characterization of two Clostridium acetobutylicum ATCC824 butanol dehydrogenase isoenzyme genes. J. Bacteriol. 174, 71497158.