• 1
    Wagner, M., Erhart, R., Manz, W., Amann, R., Lemmer, H., Wedi, D. and Schleifer, K.-H. (1994) Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge. Appl. Environ. Microbiol. 60, 792800.
  • 2
    Wagner, M., Rath, G., Amann, R., Koops, H.-P. and Schleifer, K.-H. (1995) In situ identification of ammonia-oxidizing bacteria. Syst. Appl. Microbiol. 17, 251264.
  • 3
    Wagner, M., Amann, R., Kämpfer, P., Assmus, B., Hartman, A., Hutzler, P., Springer, N. and Schleifer, K.-H. (1994) Identification and in situ detection of gram-negative filamentous bacteria in activated sludge. Syst. Appl. Microbiol. 17, 405417.
  • 4
    Moter, A., Leist, G., Rudolph, R., Schrank, K., Choi, B.-K., Wagner, M. and Göbel, U.B. (1998) Fluorescence in-situ hybridization on in resin embedded tissue sections shows spatial distribution of yet uncultured treponemes (submitted).
  • 5
    Hahn, D., Amann, R.I. and Zeyer, J. (1993) Detection of mRNA in Streptomyces cells by whole-cell hybridization with digoxigenin-labeled probes. Appl. Environ. Microbiol. 59, 27532757.
  • 6
    Hodson, R.E., Dustman, W.A., Garg, R.P. and Moran, M.A. (1995) In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities. Appl. Environ. Microbiol. 61, 40744082.
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    Hönerlage, W., Hahn, D. and Zeyer, J. (1995) Detection of mRNA of nprM in Bacillus megaterium ATCC 14581 grown in soil by whole-cell hybridization. Arch. Microbiol. 163, 235241.
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    Hurek, T., Egener, T. and Reinhold-Hurek, B. (1997) Divergence in nitrogenases of Azoarcus spp., Proteobacteria of the β subclass. J. Bacteriol. 179, 41724178.
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    Tolker-Nielsen, T., Holmstrøm, K. and Molin, S. (1997) Visualization of specific gene expression in single-cells of Salmonella typhimurium by in situ PCR. Appl. Environ. Microbiol. 63, 4195–4203.
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    Kuhn, M. and Goebel, W. (1995) Molecular studies of the virulence of Listeria monocytogenes. In: Genetic Engineering (Setlow, J.K., Ed.), Vol. 17, pp. 31–51. Plenum Press, New York.
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    Wuenscher, M.D., Köhler, S., Bubert, A., Gerike, U. and Goebel, W. (1993) The iap gene of Listeria monocytogenes is essential for cell viability and its gene product p60 has bacteriolytic activity. J. Bacteriol. 175, 34913501.
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    Bubert, A., Köhler, S. and Goebel, W. (1992) The homologous and heterologous regions within the iap gene allow genus- and species-specific identification of Listeria spp. by polymerase chain reaction. Appl. Environ. Microbiol. 58, 26252632.
  • 14
    Wang, R.F., Cao, W.-W. and Johnson, M.G. (1992) 16S rRNA-based probes and polymerase chain reaction method to detect Listeria monocytogenes cells added to foods. Appl. Environ. Microbiol. 58, 28272831.
  • 15
    Manz, W., Amann, R., Ludwig, W., Wagner, M. and Schleifer, K.-H. (1992) Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: problems and solutions. Syst. Appl. Microbiol. 15, 593600.
  • 16
    Amann, R., Krumholz, L. and Stahl, D.A. (1990) Fluorescent oligonucleotide probing of whole cells for determinative, phylogenetic and environmental studies in microbiology. J. Bacteriol. 172, 762770.
  • 17
    Amann, R. (1995) In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes. In: Molecular Microbial Ecology Manual (Akkerman, A.D.C., van Elsas, J.D. and de Bruijn, F.J., Eds.), pp. 1–15. Kluwer Academic, Dordrecht.
  • 18
    Amann, R.I., Zarda, B., Stahl, D.A. and Schleifer, K.H. (1992) Identification of individual prokaryotic cells with enzyme-labeled, rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 58, 30073011.
  • 19
    Roller, C., Wagner, M., Amann, R., Ludwig, W. and Schleifer, K.-H. (1994) In situ probing of Gram-positive bacteria with high DNA G+C content using 23S rRNA-targeted oligonucleotides. Microbiology 140, 28492858.
  • 20
    Schönhuber, W., Fuchs, B., Juretschko, S. and Amann, R. (1997) Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification. Appl. Environ. Microbiol. 63, 32683273.
  • 21
    Lema, M.W., Brown, A. and Calkins, J.H. (1994) A general method for the extraction of DNA from bacteria. J. Microbiol. Methods 18, 167172.
  • 22
    Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
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    Oelmüller, U., Krüger, N., Steinbüchel, A. and Friedrich, C.G. (1990) Isolation of prokaryotic RNA and detection of specific mRNA with biotinylated probes. J. Microbiol. Methods 11, 7384.
  • 24
    Beimfohr, C., Krause, A., Amann, R., Ludwig, W. and Schleifer, K.-H. (1993) In situ identification of Lactococci, Enterococci and Streptococci. Syst. Appl. Microbiol. 16, 450456.
  • 25
    De Los Reyes, F.L., Ritter, W. and Raskin, L. (1997) Group-specific small subunit rRNA hybridization probes to characterize filamentous foaming in activated sludge systems. Appl. Environ. Microbiol. 63, 11071117.
  • 26
    Köhler, S., Leimeister-Wächter, M., Chakraborty, T., Lottspeich, F. and Goebel, W. (1990) The gene coding for the protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes. Infect. Immun. 58, 19431950.
  • 27
    Wesendorf, M.W. and Brelje, T.C. (1992) Which fluorophore is the brightest? A comparison of the staining obtained using fluorescein, tetramethylrhodamine, lissamine rhodamine, Texas Red and cyanine 3.18. Histochemistry 98, 8185.
  • 28
    Trebesius, K., Amann, R., Ludwig, W., Mühlegger, K. and Schleifer, K.-H. (1994) Identification of whole fixed bacterial cells with nonradioactive 23S rRNA-targeted polynucleotide probes. Appl. Environ. Microbiol. 60, 32283235.