Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing

Authors

  • Michael K Winson,

    1. Food Microbiology Section, School of Biological Sciences, Division of Food Sciences, Sutton Bonington Campus, University of Nottingham, Nottingham, Leics. LE12 5RD, UK
    2. School of Pharmaceutical Sciences, University Park, University of Nottingham, Nottingham NG7 5RD, UK
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    • 1Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth SY23 3DD, UK.

  • Simon Swift,

    Corresponding author
    1. Food Microbiology Section, School of Biological Sciences, Division of Food Sciences, Sutton Bonington Campus, University of Nottingham, Nottingham, Leics. LE12 5RD, UK
    2. School of Pharmaceutical Sciences, University Park, University of Nottingham, Nottingham NG7 5RD, UK
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  • Leigh Fish,

    1. Food Microbiology Section, School of Biological Sciences, Division of Food Sciences, Sutton Bonington Campus, University of Nottingham, Nottingham, Leics. LE12 5RD, UK
    2. School of Pharmaceutical Sciences, University Park, University of Nottingham, Nottingham NG7 5RD, UK
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  • John P Throup,

    1. Food Microbiology Section, School of Biological Sciences, Division of Food Sciences, Sutton Bonington Campus, University of Nottingham, Nottingham, Leics. LE12 5RD, UK
    2. School of Pharmaceutical Sciences, University Park, University of Nottingham, Nottingham NG7 5RD, UK
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  • Frieda Jørgensen,

    1. Food Microbiology Section, School of Biological Sciences, Division of Food Sciences, Sutton Bonington Campus, University of Nottingham, Nottingham, Leics. LE12 5RD, UK
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  • Siri Ram Chhabra,

    1. School of Pharmaceutical Sciences, University Park, University of Nottingham, Nottingham NG7 5RD, UK
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  • Barrie W Bycroft,

    1. School of Pharmaceutical Sciences, University Park, University of Nottingham, Nottingham NG7 5RD, UK
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  • Paul Williams,

    1. School of Pharmaceutical Sciences, University Park, University of Nottingham, Nottingham NG7 5RD, UK
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  • Gordon S.A.B Stewart

    1. Food Microbiology Section, School of Biological Sciences, Division of Food Sciences, Sutton Bonington Campus, University of Nottingham, Nottingham, Leics. LE12 5RD, UK
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*Corresponding author. Mailing address: Institute of Infections and Immunity, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK. Tel.: +44 (115) 970 9907; Fax: +44 (115) 970 9923; E-mail: mrzsw@mrn1.nott.ac.uk

Abstract

Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.

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