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Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing

  1. Michael K Winson1,2,†,
  2. Simon Swift1,2,*,
  3. Leigh Fish1,2,
  4. John P Throup1,2,
  5. Frieda Jørgensen1,
  6. Siri Ram Chhabra2,
  7. Barrie W Bycroft2,
  8. Paul Williams2,
  9. Gordon S.A.B Stewart1

Article first published online: 17 JAN 2006

DOI: 10.1111/j.1574-6968.1998.tb13044.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 163, Issue 2, pages 185–192, June 1998

Additional Information

How to Cite

Winson, M. K., Swift, S., Fish, L., Throup, J. P., Jørgensen, F., Chhabra, S. R., Bycroft, B. W., Williams, P. and Stewart, G. S.A.B. (1998), Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing. FEMS Microbiology Letters, 163: 185–192. doi: 10.1111/j.1574-6968.1998.tb13044.x

Author Information

  1. 1

    Food Microbiology Section, School of Biological Sciences, Division of Food Sciences, Sutton Bonington Campus, University of Nottingham, Nottingham, Leics. LE12 5RD, UK

  2. 2

    School of Pharmaceutical Sciences, University Park, University of Nottingham, Nottingham NG7 5RD, UK

  1. †

    1Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth SY23 3DD, UK.

* *Corresponding author. Mailing address: Institute of Infections and Immunity, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK. Tel.: +44 (115) 970 9907; Fax: +44 (115) 970 9923; E-mail: mrzsw@mrn1.nott.ac.uk

  1. †

    1Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth SY23 3DD, UK.

Publication History

  1. Issue published online: 17 JAN 2006
  2. Article first published online: 17 JAN 2006
  3. Received 30 January 1998, Revised 20 April 1998, Accepted 23 April 1998

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Keywords:

  • Bioluminescence;
  • lux;
  • Quorum sensing;
  • N-Acyl homoserine lactone

Abstract

Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.

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