1Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth SY23 3DD, UK.
Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing
Article first published online: 17 JAN 2006
FEMS Microbiology Letters
Volume 163, Issue 2, pages 185–192, June 1998
How to Cite
Winson, M. K., Swift, S., Fish, L., Throup, J. P., Jørgensen, F., Chhabra, S. R., Bycroft, B. W., Williams, P. and Stewart, G. S.A.B. (1998), Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing. FEMS Microbiology Letters, 163: 185–192. doi: 10.1111/j.1574-6968.1998.tb13044.x
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- Received 30 January 1998, Revised 20 April 1998, Accepted 23 April 1998
- Quorum sensing;
- N-Acyl homoserine lactone
Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.