Rapid identification of human intestinal bifidobacteria by 16S rRNA-targeted species- and group-specific primers

Authors

  • Takahiro Matsuki,

    Corresponding author
    1. Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186, Japan
    2. Graduate School of Agriculture and Agricultural Life Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
    Search for more papers by this author
  • Koichi Watanabe,

    1. Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186, Japan
    Search for more papers by this author
  • Ryuichiro Tanaka,

    1. Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186, Japan
    Search for more papers by this author
  • Hiroshi Oyaizu

    1. Graduate School of Agriculture and Agricultural Life Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
    Search for more papers by this author

*Corresponding author. Tel.: +81 (42) 577 8960; Fax: +81 (42) 577 3020; E-mail: aa0901@hongo.ecc.u-tokyo.ac.jp

Abstract

On the basis of 16S rRNA sequences, species- and group-specific primers for Bifidobacterium adolescentis, B. angulatum, B. bifidum, B. breve, the B. catenulatum group (B. catenulatum and B. pseudocatenulatum), and the B. longum group (B. longum and B. infantis), which are species commonly found in human intestinal tracts, were developed. The specificity of these primers was confirmed through the use of DNA extracted from 46 strains of 31 Bifidobacterium species, as well as 14 non-bifidobacterial species that are the predominant bacteria in the human intestinal tract. The present species-specific primers were applied to the identification of 43 isolated strains, consisting of six strains of B. adolescentis, eight of the B. catenulatum group, seven of B. bifidum, nine of B. breve, and 13 of the B. longum group.

Ancillary