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The oxyR gene from Erwinia carotovora: cloning, sequence analysis and expression in Escherichia coli
Article first published online: 17 JAN 2006
DOI: 10.1111/j.1574-6968.1998.tb13242.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
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How to Cite
Calcutt, M. J., Lewis, M. S. and Eisenstark, A. (1998), The oxyR gene from Erwinia carotovora: cloning, sequence analysis and expression in Escherichia coli. FEMS Microbiology Letters, 167: 295–301. doi: 10.1111/j.1574-6968.1998.tb13242.x
Publication History
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- Received 22 June 1998, Revised 21 August 1998, Accepted 22 August 1998
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Keywords:
- Hydrogen peroxide resistance;
- OxyR;
- Erwinia carotovora
Abstract
Homologs of the Escherichia coli oxyR gene were identified in several Erwinia species, using a combination of PCR and Southern hybridization analysis. The oxyR gene from Erwinia carotovora was isolated on a cosmid clone and characterized. The gene and deduced gene product shared high level sequence identity with their E. coli counterparts (78 and 89% identity, respectively). In E. coli, the oxyR gene is a transcriptional activator that, under oxidizing conditions, induces expression of a set of oxidative defence genes. OxyR null mutants are, therefore, sensitive to hydrogen peroxide. Introduction of the E. carotovora oxyR gene into an E. coli oxyR mutant resulted in transformants that were hydrogen peroxide resistant, indicating that the Erwinia protein was functional in E. coli.