1Asahi Chemical Industry Co. Ltd, 632-1 Mifuku, Ohito, Tagata, Shizuoka 410-23, Japan.
The DrrC protein of Streptomyces peucetius, a UvrA-like protein, is a DNA-binding protein whose gene is induced by daunorubicin
Article first published online: 17 JAN 2006
FEMS Microbiology Letters
Volume 168, Issue 2, pages 243–249, November 1998
How to Cite
Furuya, K. and Richard Hutchinson, C. (1998), The DrrC protein of Streptomyces peucetius, a UvrA-like protein, is a DNA-binding protein whose gene is induced by daunorubicin. FEMS Microbiology Letters, 168: 243–249. doi: 10.1111/j.1574-6968.1998.tb13280.x
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- Received 23 July 1998, Revised 5 September 1998, Accepted 15 September 1998
- Antibiotic resistance;
- Antitumor antibiotic;
- DNA binding protein;
- Streptomyces peucetius
DrrC, a daunorubicin resistance protein with a strong sequence similarity to the UvrA protein involved in excision repair of DNA, is induced by daunorubicin in Streptomyces peucetius and behaves like an ATP-dependent, DNA binding protein in vitro. The refolded protein obtained from expression of the drrC gene in Escherichia coli was used to conduct gel retardation assays. DrrC bound a DNA segment containing the promoter region of a daunorubicin production gene only in the presence of ATP and daunorubicin. This result suggests that DrrC is a novel type of drug self-resistance protein with DNA binding properties like those of UvrA. Western blotting analysis with a polyclonal antiserum generated against His-tagged DrrC showed that the appearance of DrrC in S. peucetius is coincident with the onset of daunorubicin production and that the drrC gene is induced by daunorubicin. These data also showed that the DnrN and DnrI regulatory proteins are required for drrC expression. The level of DrrA, another daunorubicin resistance protein that resembles ATP-dependent bacterial antiporters, was regulated in the same way as that of DrrC.