The 105-kDa protein component of Bacillus cereus non-haemolytic enterotoxin (Nhe) is a metalloprotease with gelatinolytic and collagenolytic activity

Authors

  • Terje Lund,

    Corresponding author
    1. Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033 Oslo, Norway
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  • Per Einar Granum

    1. Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033 Oslo, Norway
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*Corresponding author. Tel: +47 (22) 59 70 33; Fax: +47 (22) 96 48 50, E-mail address: terje.lund@veths.no

Abstract

A sequence of 91 amino acids residues, probably starting from the N-terminal of the mature protein, was determined for the 105-kDa protein of the non-haemolytic enterotoxin of Bacillus cereus. The last part of this sequence was similar to parts of the N-terminal portions of two collagenases of Clostridium histolyticum and Clostridium perfringens. Zymography, with intact collagen fibril and gelatin as substrates, showed that the 105-kDa protein had collagenolytic and gelatinolytic activity. The 105-kDa protein also showed activity against a typical collagenase substrate, azocoll, and was inhibited by EDTA and 1,10-phenanthroline. We conclude that the 105-kDa protein is a collagenase.

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