1574-6968/asset/FML_left.gif?v=1&s=d9ad90a5f75253894fe5059aa2f75bf910ebf83a)
1574-6968/asset/FML_right.gif?v=1&s=48d5e33deef512c09651020f71074ad93d3351e7)
Cloning and complete nucleotide sequence of Acinetobacter radioresistens CMC-1 AglyA gene encoding serine hydroxymethyltransferase
Article first published online: 17 JAN 2006
DOI: 10.1111/j.1574-6968.1999.tb13402.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
Additional Information
How to Cite
Hong, M. C., Wu, M. L. and Chang, M. C. (1999), Cloning and complete nucleotide sequence of Acinetobacter radioresistens CMC-1 AglyA gene encoding serine hydroxymethyltransferase. FEMS Microbiology Letters, 170: 413–418. doi: 10.1111/j.1574-6968.1999.tb13402.x
Publication History
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- Received 15 September 1998, Revised 16 November 1998, Accepted 27 November 1998
- Abstract
- Article
- References
- Cited By
Keywords:
- Acinetobacter radioresistens;
- Serine hydroxymethyltransferase;
- C1 unit
Abstract
A gene (AglyA) encoding serine hydroxymethyltransferase of Acinetobacter radioresistens CMC-1 was cloned and sequenced. Nucleotide sequence analysis of AglyA predicted a single open reading frame (ORF) of 1251 bp encoding a 417-amino acid polypeptide. Two putative MetR-like binding sites (5′-TGAAACATGAGCT) and (5′-TGAGCAAAGTTCA), centered at bp −123 and −95 relative to the +1 translation start site were found, which have six out of nine and eight out of nine nucleotides that match to the consensus sequence of Escherichia coli (5′-TGAANNT/ANNTTCA), respectively. The enzyme also showed a high level of homology to other sources of serine hydroxymethyltransferase proteins.