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Characterisation of strain-specific sequences from an abortifacient strain of ovine Chlamydia psittaci using subtraction hybridisation

  1. Julie L Creelan1,
  2. Anthony J Bjourson2,
  3. Brian M Meehan3,
  4. Samuel J McCullough1,*

Article first published online: 17 JAN 2006

DOI: 10.1111/j.1574-6968.1999.tb13407.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 171, Issue 1, pages 17–25, February 1999

Additional Information

How to Cite

Creelan, J. L., Bjourson, A. J., Meehan, B. M. and McCullough, S. J. (1999), Characterisation of strain-specific sequences from an abortifacient strain of ovine Chlamydia psittaci using subtraction hybridisation. FEMS Microbiology Letters, 171: 17–25. doi: 10.1111/j.1574-6968.1999.tb13407.x

Author Information

  1. 1

    Veterinary Sciences Division, Department of Agriculture for Northern Ireland, The Queen's University of Belfast, Stormont, Belfast BT4 3SD, UK

  2. 2

    Food and Agricultural Microbiology Research Division, Department of Agriculture for Northern Ireland, Newforge Lane, Belfast BT9 5PX, UK

  3. 3

    Department of Veterinary Science, The Queen's University of Belfast, Stormont, Belfast BT4 3SD, UK

* *Corresponding author. Tel.: +44 (1232) 525630; Fax: +44 (1232) 525731; E-mail: sam.mcmullough@dani.gov.uk

Publication History

  1. Issue published online: 17 JAN 2006
  2. Article first published online: 17 JAN 2006
  3. Received 17 September 1998, Revised 27 November 1998, Accepted 3 December 1998

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Keywords:

  • Chlamydia psittaci;
  • Ovine abortion;
  • Subtraction hybridization;
  • Strain-specific probe;
  • Helicase

Abstract

Enzootic abortion in ewes (EAE) is caused by strains of Chlamydia psittaci which have the ability to invade and colonise the placenta of sheep. In an attempt to improve diagnostic methods for the detection of EAE, subtraction hybridisation was used to isolate unique fragments of the genome of an abortifacient strain (S26/3) of C. psittaci. One S26/3 strain-specific sequence identified was shown to encode a putative helicase which is repeated throughout the EAE genome. The labelled strain-specific helicase gene fragment was used in a dot-blot hybridisation test for the detection of EAE DNA in ovine placental samples. We report the identification of C. psittaci S26/3 strain-specific sequences with potential as diagnostic probes for the detection of EAE.

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