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Detection of methanogenic archaea in seawater particles and the digestive tract of a marine fish species

  1. Marc J.E.C. van der Maarel1,*,
  2. Wander Sprenger2,
  3. René Haanstra1,
  4. Larry J. Forney1

Article first published online: 17 JAN 2006

DOI: 10.1111/j.1574-6968.1999.tb13501.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 173, Issue 1, pages 189–194, April 1999

Additional Information

How to Cite

van der Maarel, M. J., Sprenger, W., Haanstra, R. and Forney, L. J. (1999), Detection of methanogenic archaea in seawater particles and the digestive tract of a marine fish species. FEMS Microbiology Letters, 173: 189–194. doi: 10.1111/j.1574-6968.1999.tb13501.x

Author Information

  1. 1

    Laboratory of Microbial Ecology, Center for Ecological and Evolutionary Studies, University of Groningen, P.O. Box 14, 9750 AA Haren, Netherlands

  2. 2

    Laboratory for Microbiology and Evolutionary Biology, University of Nijmegen, Nijmegen, Netherlands

* Corresponding author. Tel.: +31 (50) 363-2236; Fax: +31 (50) 363-2154; E-mail: maarelmj@biol.rug.nl

Publication History

  1. Issue published online: 17 JAN 2006
  2. Article first published online: 17 JAN 2006
  3. Received 9 September 1998, Revised 15 December 1998, Accepted 30 January 1999

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Keywords:

  • Methanogenic archaea;
  • Seawater particle;
  • Fish feces;
  • Intestinal microflora;
  • 16S rDNA;
  • PCR

Abstract

A methanogen-specific nested PCR approach was used to detect methanogenic archaea in seawater particles of the North Sea and the feces and the digestive tract of flounder (Platichthys flesus), a fish found in the North Sea. A number of 16S rDNA sequences with 97.6–99.5% similarity to Methanococcoides methylutens were found in the seawater particles as well as the digestive tract and fecal samples.

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