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Nitrogen regulation in Corynebacterium glutamicum: isolation of genes involved and biochemical characterization of corresponding proteins
Article first published online: 17 JAN 2006
DOI: 10.1111/j.1574-6968.1999.tb13518.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
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How to Cite
Jakoby, M., Krämer, R. and Burkovski, A. (1999), Nitrogen regulation in Corynebacterium glutamicum: isolation of genes involved and biochemical characterization of corresponding proteins. FEMS Microbiology Letters, 173: 303–310. doi: 10.1111/j.1574-6968.1999.tb13518.x
Publication History
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- Received 23 November 1998, Revised 9 February 1999, Accepted 10 February 1999
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Keywords:
- Nitrogen control;
- Gram-positive bacterium;
- Actinomycete;
- Mycobacterium
Abstract
The regulation of nitrogen assimilation was investigated in the Gram-positive actinomycete Corynebacterium glutamicum. Biochemical studies and site-directed mutagenesis revealed that glutamine synthetase activity is regulated via adenylylation in this organism. The genes encoding the central signal transduction protein PII (glnB) and the primary nitrogen sensor uridylyltransferase (glnD) were isolated and sequenced. Additionally, genes putatively involved in the degradation of ornithine (ocd) and sarcosine (soxA), ammonium uptake (amtP) and protein secretion (ftsY, srp) were identified in C. glutamicum. Based on these observations, the mechanism of N regulation in C. glutamicum is similar to that of the Gram-negative Escherichia coli. As deduced from data base searches, the described regulation may also hold true for the important pathogen Mycobacterium glutamicum.