Production of a recombinant lipase artificially localized on the Bacillus subtilis cell surface

Authors

  • Atsushi Tsuchiya,

    1. Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano 386-8567, Japan
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  • Gota Kobayashi,

    1. Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano 386-8567, Japan
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  • Hiroki Yamamoto,

    1. Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano 386-8567, Japan
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  • Junichi Sekiguchi

    Corresponding author
    1. Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano 386-8567, Japan
      *Corresponding author. Tel.: +81 (268) 21 5344; Fax: +81 (268) 21 5331, E-mail address: jsekigu@giptc.shinshu-u.ac.jp
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*Corresponding author. Tel.: +81 (268) 21 5344; Fax: +81 (268) 21 5331, E-mail address: jsekigu@giptc.shinshu-u.ac.jp

Abstract

The lipB gene encoding an extracellular lipase, LipB, from Bacillus subtilis was fused to the 5′-terminal region of the cwlB gene encoding the cell wall-binding domain of the major cell wall hydrolase (autolysin). SDS-polyacrylamide gel electrophoresis and zymography revealed that the fused protein is localized in the B. subtilis cell wall fraction and retains lipase activity.

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