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Production of a recombinant lipase artificially localized on the Bacillus subtilis cell surface
Article first published online: 17 JAN 2006
DOI: 10.1111/j.1574-6968.1999.tb13686.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
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How to Cite
Tsuchiya, A., Kobayashi, G., Yamamoto, H. and Sekiguchi, J. (1999), Production of a recombinant lipase artificially localized on the Bacillus subtilis cell surface. FEMS Microbiology Letters, 176: 373–378. doi: 10.1111/j.1574-6968.1999.tb13686.x
Publication History
- Issue published online: 17 JAN 2006
- Article first published online: 17 JAN 2006
- Received 4 May 1999, Revised 26 May 1999, Accepted 26 May 1999
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Keywords:
- Bacillus subtilis;
- Lipase;
- Autolysin;
- Surface engineering
Abstract
The lipB gene encoding an extracellular lipase, LipB, from Bacillus subtilis was fused to the 5′-terminal region of the cwlB gene encoding the cell wall-binding domain of the major cell wall hydrolase (autolysin). SDS-polyacrylamide gel electrophoresis and zymography revealed that the fused protein is localized in the B. subtilis cell wall fraction and retains lipase activity.