Can the grey mould disease of the grape-vine be controlled by yeast?
Article first published online: 9 JAN 2006
FEMS Microbiology Letters
Volume 189, Issue 2, pages 233–237, August 2000
How to Cite
Masih, E. I., Alie, I. and Paul, B. (2000), Can the grey mould disease of the grape-vine be controlled by yeast?. FEMS Microbiology Letters, 189: 233–237. doi: 10.1111/j.1574-6968.2000.tb09236.x
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 16 June 2000, Revised 21 June 2000, Accepted 23 June 2000
- Biological control;
- Nuclear ribosomal DNA;
- Pichia anomala;
- Botrytis cinerea
Botrytis cinerea has been found to be highly pathogenic to ‘Chardonnay’ and ‘Pinot noir’ cultivars of the grape-vine producing the characteristic grey mould symptoms within 7 days of inoculation to the vitro-plants. The yeast Pichia anomala (strain FY-102), isolated from apple skin, was found to be antagonistic to B. cinerea as it completely inhibited the appearance of the grey mould symptoms when grown together. The yeast was responsible for morphological changes such as coagulation and leakage of the cytoplasm of B. cinerea. The pathogen, when applied together with P. anomala, failed to bring about the grey mould symptoms on the grape-vine, suggesting that the yeast could control the expression of this disease. An account of the interaction between B. cinerea and P. anomala, as well as the sequences of the complete ITS region of the ribosomal DNA of the yeast are described here.