The predatory soil flagellate Heteromita globosa stimulates toluene biodegradation by a Pseudomonas sp.
Article first published online: 9 JAN 2006
FEMS Microbiology Letters
Volume 194, Issue 1, pages 39–45, January 2001
How to Cite
Mattison, R. G. and Harayama, S. (2001), The predatory soil flagellate Heteromita globosa stimulates toluene biodegradation by a Pseudomonas sp. FEMS Microbiology Letters, 194: 39–45. doi: 10.1111/j.1574-6968.2001.tb09443.x
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 21 August 2000, Revised 10 October 2000, Accepted 10 October 2000
- Carbon 14;
A model food chain was established to investigate the influence of grazing by flagellates on bacteria degrading toluene in batch culture. The rate of toluene consumed by a Pseudomonas sp. strain PS+ (max. 0.37 fmol cell−1 h−1) was significantly higher in the presence of the bacterivorous flagellate Heteromita globosa (max. 1.38 fmol cell−1 h−1). A maximum increase of up to 7.5 times was observed in the rate of toluene consumed by these bacteria during exponential growth of this flagellate. Carbon conversion efficiency (CCE) of bacteria to flagellate biomass was estimated to be 33.4% based on measured biovolumes and published values for carbon contents. However, the CCE for toluene-derived carbon was lower (max. 4.9%) when calculations were based on incorporation of [ring-U-14C]toluene into biomass of flagellates grazing on labelled bacteria. The findings suggest a potential role for flagellates in bioremediation processes.