Characterization of the common nodulation genes of the photosynthetic Bradyrhizobium sp. ORS285 reveals the presence of a new insertion sequence upstream of nodA

Authors

  • Clemence Chaintreuil,

    1. Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD, CIRAD, AGRO-M, INRA TA 10/J, Campus de Baillarguet, 34398 Montpellier Cedex 5, France
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  • Catherine Boivin,

    1. Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD, CIRAD, AGRO-M, INRA TA 10/J, Campus de Baillarguet, 34398 Montpellier Cedex 5, France
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  • Bernard Dreyfus,

    1. Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD, CIRAD, AGRO-M, INRA TA 10/J, Campus de Baillarguet, 34398 Montpellier Cedex 5, France
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  • Eric Giraud

    Corresponding author
    1. Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD, CIRAD, AGRO-M, INRA TA 10/J, Campus de Baillarguet, 34398 Montpellier Cedex 5, France
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*Corresponding author. Tel.: +33 (467) 593783; Fax: +33 (467) 593802, E-mail address: giraud@mpl.ird.fr

Abstract

We isolated and characterized nodA genes from photosynthetic and non-photosynthetic rhizobia nodulating the legume genus Aeschynomene, and found that the nodA sequence from photosynthetic stem-nodulating bacteria was phylogenetically distant from the other already described nodA genes. Characterization of the photosynthetic strain ORS285 common nod gene cluster (nodABC) showed, upstream of nodA, the presence of a new insertion sequence element belonging to the IS3 family and specific to a group of photosynthetic strains from Aeschynomene.

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