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The mtl genes and the mannitol-1-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026
Article first published online: 9 JAN 2006
DOI: 10.1111/j.1574-6968.2001.tb09473.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
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How to Cite
Otte, S. and Lengeler, J. W. (2001), The mtl genes and the mannitol-1-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026. FEMS Microbiology Letters, 194: 221–227. doi: 10.1111/j.1574-6968.2001.tb09473.x
Publication History
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 1 September 2000, Revised 28 November 2000, Accepted 28 November 2000
- Abstract
- Article
- References
- Cited By
Keywords:
- mtl gene;
- Mannitol;
- Mannitol-1-phosphate dehyrogenase;
- Klebsiella pneumoniae
Abstract
The mtl operon of Klebsiella pneumoniae KAY2026 (formerly Aerobacter aerogenes 1033-5P14) was shown to contain as the promoter-proximal gene mtlA, encoding a D-mannitol-specific enzyme II transporter (IICBAMtl). This gene is followed by mtlD, coding for a mannitol-1-phosphate dehydrogenase (MtlD, 382 amino acid residues), and mtlR (MtlR, 195 amino acid residues) coding for a putative repressor, gene mtlR overlaps the termination codon of mtlD. The DNA and protein sequences are highly similar to the corresponding genes (81% identical bp) and proteins (79–85% identical amino acids) of Escherichia coli K-12. A truncated form of MtlD lacking the 162 C-terminal amino acid residues still shows 10% dehydrogenase activity which may explain the controversy in the literature concerning the properties of mannitol-phosphate and other medium-length dehydrogenases.