Polymerase chain reaction assay for the detection of Bacillus cereus group cells

Authors

  • Bjarne Munk Hansen,

    1. Department of Microbial Ecology and Biotechnology, National Environmental Research Institute, P.O. Box 358, Frederiksborgvej 399, DK-4000 Roskilde, Denmark
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  • Thomas D. Leser,

    1. Danish Veterinary Laboratory, Ministry of Food, Agriculture and Fisheries, DK-1790 Copenhagen V, Denmark
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  • Niels Bohse Hendriksen

    Corresponding author
    1. Department of Microbial Ecology and Biotechnology, National Environmental Research Institute, P.O. Box 358, Frederiksborgvej 399, DK-4000 Roskilde, Denmark
      *Corresponding author. Tel.: +45 (46) 30 13 72; Fax: +45 (46) 30 12 16, E-mail: nbh@dmu.dk
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*Corresponding author. Tel.: +45 (46) 30 13 72; Fax: +45 (46) 30 12 16, E-mail: nbh@dmu.dk

Abstract

Recent investigations have shown that members of the Bacillus cereus group carry genes which have the potential to cause gastrointestinal and somatic diseases. Although most cases of diseases caused by the B. cereus group bacteria are relatively mild, it is desirable to be able to detect members of the B. cereus group in food and in the environment. Using 16S rDNA as target, a PCR assay for the detection of B. cereus group cells has been developed. Primers specific for the 16S rDNA of the B. cereus group bacteria were selected and used in combination with consensus primers for 16S rDNA as internal PCR procedure control. The PCR procedure was optimized with respect to annealing temperature. When DNA from the B. cereus group bacteria was present, the PCR assay yielded a B. cereus specific fragment, while when non-B. cereus prokaryotic DNA was present, the consensus 16S rDNA primers directed synthesis of the PCR products. The PCR analyses with DNA from a number of non-B. cereus confirmed the specificity of the PCR assay.

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