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Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge
Article first published online: 9 JAN 2006
DOI: 10.1111/j.1574-6968.2001.tb10963.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
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How to Cite
Lu, J., Nogi, Y. and Takami, H. (2001), Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiology Letters, 205: 291–297. doi: 10.1111/j.1574-6968.2001.tb10963.x
Publication History
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 6 August 2001, Revised 21 October 2001, Accepted 21 October 2001
- Abstract
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Keywords:
- Deep-sea isolate;
- Halotolerant alkaliphile;
- 16S rDNA sequence;
- DNA–DNA hybridization;
- Oceanobacillus iheyensis
Abstract
An extremely halotolerant and alkaliphilic bacterium was isolated previously from deep-sea sediment collected at a depth of 1050 m on the Iheya Ridge. The strain, designated HTE831 (JCM 11309, DSM 14371), was Gram-positive, strictly aerobic, rod-shaped, motile by peritrichous flagella, and spore-forming. Strain HTE831 grew at salinities of 0–21% (w/v) NaCl at pH 7.5 and 0–18% at pH 9.5. The optimum concentration of NaCl for growth was 3% at both pH 7.5 and 9.5. The G+C content of its DNA was 35.8%.
Low level (12–30%) of DNA–DNA relatedness between strain HTE831 and the species of these genera was found, indicating that HTE831 could not be classified as a member of a new species belonging to known genera. Based on phylogenetic analysis using 16S rDNA sequencing, chemotaxonomy, and the physiology of strain HTE831, it is proposed that this organism is a member of a new species in a new genus, for which the name Oceanobacillus iheyensis is proposed.