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An IS900-like sequence found in a Mycobacterium sp. other than Mycobacterium avium subsp. paratuberculosis
Article first published online: 9 JAN 2006
DOI: 10.1111/j.1574-6968.2002.tb11142.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
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How to Cite
Englund, S., Bölske, G. and Johansson, K.-E. (2002), An IS900-like sequence found in a Mycobacterium sp. other than Mycobacterium avium subsp. paratuberculosis. FEMS Microbiology Letters, 209: 267–271. doi: 10.1111/j.1574-6968.2002.tb11142.x
Publication History
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 5 January 2002, Revised 12 February 2002, Accepted 12 February 2002
- Abstract
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Keywords:
- Diagnosis;
- IS900;
- Polymerase chain reaction;
- Mycobacterium avium subsp. paratuberculosis
Abstract
The insertion sequence IS900 has been considered specific for Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) and has, therefore, been used as the target gene for diagnostic PCR of M. paratuberculosis. From a healthy dairy cow we have isolated and characterised a mycobacterium harbouring one copy of a sequence with 94% identity to IS900 at the nucleic acid level. The isolate was shown to be related to Mycobacterium cookii, as assessed by 16S rRNA sequencing. Strong amplifications were obtained with several PCR primers described for detection of IS900. This finding shows the need of alternative PCR systems based on other genes than IS900 to confirm the presence of M. paratuberculosis.