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Pseudomonas aeruginosa galU is required for a complete lipopolysaccharide core and repairs a secondary mutation in a PA103 (serogroup O11) wbpM mutant
Article first published online: 9 JAN 2006
FEMS Microbiology Letters
Volume 210, Issue 2, pages 277–283, May 2002
How to Cite
Dean, C. R. and Goldberg, J. B. (2002), Pseudomonas aeruginosa galU is required for a complete lipopolysaccharide core and repairs a secondary mutation in a PA103 (serogroup O11) wbpM mutant. FEMS Microbiology Letters, 210: 277–283. doi: 10.1111/j.1574-6968.2002.tb11193.x
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 18 January 2002, Revised 18 March 2002, Accepted 19 March 2002
- O antigen;
- Core oligosaccharide;
Insertional inactivation of wbpM in Pseudomonas aeruginosa serogroup O11 strain PA103 resulted in mutants exhibiting three distinct lipopolysaccharide (LPS) phenotypes. One mutant, PA103 wbpM-C, had a truncated LPS core and lacked O antigen. These defects were not complemented by the cloned wbpM gene, suggesting a secondary mutation was present. When the wild-type galU gene was introduced into strain PA103 wbpM-C containing the cloned wbpM gene, both LPS defects were corrected. Construction of galU mutants in P. aeruginosa serogroups O11, O5, O6 and O17 strains led to truncation of the LPS core, indicating the involvement of GalU in P. aeruginosa LPS core synthesis.