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Glutamate dehydrogenase of Halobacterium salinarum: evidence that the gene sequence currently assigned to the NADP+-dependent enzyme is in fact that of the NAD+-dependent glutamate dehydrogenase

  1. Bronagh M. Hayden1,
  2. María-José Bonete2,
  3. Paul E. Brown3,
  4. Arthur J.G. Moir3,
  5. Paul C. Engel1,*

Article first published online: 9 JAN 2006

DOI: 10.1111/j.1574-6968.2002.tb11200.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 211, Issue 1, pages 37–41, May 2002

Additional Information

How to Cite

Hayden, B. M., Bonete, M.-J., Brown, P. E., Moir, A. J. and Engel, P. C. (2002), Glutamate dehydrogenase of Halobacterium salinarum: evidence that the gene sequence currently assigned to the NADP+-dependent enzyme is in fact that of the NAD+-dependent glutamate dehydrogenase. FEMS Microbiology Letters, 211: 37–41. doi: 10.1111/j.1574-6968.2002.tb11200.x

Author Information

  1. 1

    Department of Biochemistry and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland

  2. 2

    Department of Biochemistry, University of Alicante, Alicante, Spain

  3. 3

    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK

* *Corresponding author: Tel.: +353 (1) 716 15 47; Fax: +353 (1) 283 72 11, E-mail address: paul.engel@ucd.ie

Publication History

  1. Issue published online: 9 JAN 2006
  2. Article first published online: 9 JAN 2006
  3. Received 13 February 2002, Revised 25 March 2002, Accepted 26 March 2002

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Keywords:

  • Halophile;
  • Glutamate dehydrogenase;
  • Coenzyme specificity;
  • Gene sequence;
  • Peptide sequence

Abstract

A GDH gene from Halobacterium salinarum has been cloned and sequenced and the publication assigns the sequence to the NADP+-glutamate dehydrogenase of this organism. We have expressed this gene in Escherichia coli and find that it encodes an NAD+-dependent glutamate dehydrogenase without activity towards NADP+. Further, peptide sequence from the two corresponding proteins supports the view that the deposited sequence is indeed that of the NAD+-dependent glutamate dehydrogenase. Sequence from the NAD+-dependent protein matches the published gene sequence, whereas sequence from the NADP+ glutamate dehydrogenase does not.

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