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Development of a bipartite method for Fusarium identification based on cellobiohydrolase-C: CAPS and Western blot analysis

  1. Didier Hatsch,
  2. Vincent Phalip*,
  3. Jean-Marc Jeltsch

Article first published online: 9 JAN 2006

DOI: 10.1111/j.1574-6968.2002.tb11313.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 213, Issue 2, pages 245–249, August 2002

Additional Information

How to Cite

Hatsch, D., Phalip, V. and Jeltsch, J.-M. (2002), Development of a bipartite method for Fusarium identification based on cellobiohydrolase-C: CAPS and Western blot analysis. FEMS Microbiology Letters, 213: 245–249. doi: 10.1111/j.1574-6968.2002.tb11313.x

Author Information

  1. U.M.R. 7100, Université Louis Pasteur–CNRS, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brant, 67400 Illkirch-Graffenstaden, France

* *Corresponding author. Tel.: +33 3 90 24 47 94; Fax: +33 3 90 24 46 83, E-mail address: vincent.phalip@esbs.u-strasbg.fr

Publication History

  1. Issue published online: 9 JAN 2006
  2. Article first published online: 9 JAN 2006
  3. Received 19 March 2002, Revised 21 June 2002, Accepted 21 June 2002

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Keywords:

  • Cellobiohydrolase;
  • Cleaved amplified polymorphic sequence;
  • Immunodetection;
  • Fusarium identification

Abstract

The identification of 12 Fusarium strains isolated from diseased hops (Humulus lupulus, L.) was achieved by a strategy based on cellobiohydrolase-C: cleaved amplified polymorphic sequence analysis targeting the gene and the use of an antibody directed against a peptide of the Fusarium graminearum enzyme. This strategy is shown to be rapid and reliable for all the Fusarium of our collection: F. avenaceum, F. graminearum, F. sambucinum, F. sporotrichioides, F. tricinctum and F. venenatum.

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