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Cellulomonas flavigena: characterization of an endo-1,4-xylanase tightly induced by sugarcane bagasse
Article first published online: 9 JAN 2006
DOI: 10.1111/j.1574-6968.2002.tb11348.x
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
Additional Information
How to Cite
Mayorga-Reyes, L., Morales, Y., Salgado, L. M., Ortega, A. and Ponce-Noyola, T. (2002), Cellulomonas flavigena: characterization of an endo-1,4-xylanase tightly induced by sugarcane bagasse. FEMS Microbiology Letters, 214: 205–209. doi: 10.1111/j.1574-6968.2002.tb11348.x
Publication History
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 20 February 2002, Revised 17 July 2002, Accepted 18 July 2002
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Keywords:
- Cellulomonas flavigena;
- Xylanase;
- Transcriptional regulation;
- Sugarcane bagasse
Abstract
Xylanases, an important group of enzymes for biomass degradation in the industry, are commonly found forming complex multienzyme systems. As a preliminary step to the construction of efficient xylanase producers using genetic engineering, we have characterized a gene encoding an endo-β-1,4 xylanase (xyncflA) from Cellulomonas flavigena. The xylanase activity and the xyncflA synthesis were higher when C. flavigena was grown on sugarcane bagasse. In this substrate, both activity and transcript increased with approximately the same rate during the culture period. When C. flavigena grew on glucose, low signal of mRNA was observed, suggesting that the xyncflA gene is regulated at the transcriptional level.