Sulfur-selective desulfurization of dibenzothiophene and diesel oil by newly isolated Rhodococcus sp. strains

Authors

  • Gladys Castorena,

    1. Instituto Mexicano del Petróleo, Programa de Investigación y Desarrollo en Biotecnología del Petróleo, Eje Central Lázaro Cárdenas 152, Col. San Bartolo Atepehuacan, 07730 Mexico City, DF, Mexico
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  • Claudia Suárez,

    1. Instituto Mexicano del Petróleo, Programa de Investigación y Desarrollo en Biotecnología del Petróleo, Eje Central Lázaro Cárdenas 152, Col. San Bartolo Atepehuacan, 07730 Mexico City, DF, Mexico
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  • Idania Valdez,

    1. Instituto Mexicano del Petróleo, Programa de Investigación y Desarrollo en Biotecnología del Petróleo, Eje Central Lázaro Cárdenas 152, Col. San Bartolo Atepehuacan, 07730 Mexico City, DF, Mexico
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  • Guadalupe Amador,

    1. Instituto Mexicano del Petróleo, Programa de Investigación y Desarrollo en Biotecnología del Petróleo, Eje Central Lázaro Cárdenas 152, Col. San Bartolo Atepehuacan, 07730 Mexico City, DF, Mexico
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  • Luis Fernández,

    1. Instituto Mexicano del Petróleo, Programa de Investigación y Desarrollo en Biotecnología del Petróleo, Eje Central Lázaro Cárdenas 152, Col. San Bartolo Atepehuacan, 07730 Mexico City, DF, Mexico
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  • Sylvie Le Borgne

    Corresponding author
    1. Instituto Mexicano del Petróleo, Programa de Investigación y Desarrollo en Biotecnología del Petróleo, Eje Central Lázaro Cárdenas 152, Col. San Bartolo Atepehuacan, 07730 Mexico City, DF, Mexico
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*Corresponding author. Tel.: +52 (55) 30 03 73 23; Fax: +52 (55) 30 03 77 05, E-mail address: slborgne@imp.mx

Abstract

New desulfurizing bacteria able to convert dibenzothiophene into 2-hydroxybiphenyl and sulfate were isolated from contaminated soils collected in Mexican refineries. Random amplified polymorphic DNA analysis showed they were different from previously reported Rhodococcus erythropolis desulfurizing strains. According to 16S rRNA gene sequencing and fatty acid analyses, these new isolates belonged to the genus Rhodococcus. These strains could desulfurize 4,6-dimethyldibenzothiophene which is one of the most difficult dibenzothiophene derivatives to remove by hydrodesulfurization. A deeply hydrodesulfurized diesel oil containing significant amounts of 4,6-dimethyldibenzothiophene was treated with Rhodococcus sp. IMP-S02 cells. Up to 60% of the total sulfur was removed and all the 4,6-dimethyldibenzothiophene disappeared as a result of this treatment.

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