Editor: Aharon Oren
A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8T: cloning and molecular characterization
Article first published online: 5 JAN 2006
FEMS Microbiology Letters
Volume 255, Issue 1, pages 89–95, February 2006
How to Cite
Yang, L. F., Jiang, J. Q., Zhao, B. S., Zhang, B., Feng, D. Q., Lu, W. D., Wang, L. and Yang, S. S. (2006), A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8T: cloning and molecular characterization. FEMS Microbiology Letters, 255: 89–95. doi: 10.1111/j.1574-6968.2005.00055.x
- Issue published online: 5 JAN 2006
- Article first published online: 5 JAN 2006
- Received 26 September 2005; revised 15 November 2005; accepted 15 November 2005.
- Halobacillus dabanensis;
- moderately halophilic bacterium;
- Na+/H+ antiporter;
A gene encoding a Na+/H+ antiporter was cloned from a chromosomal DNA of Halobacillus dabanensis strain D-8T by functional complementation. Its presence enabled the antiporter-deficient Escherichia coli strain KNabc to survive in the presence of 0.2 M NaCl or 5 mM LiCl. The gene was sequenced and designated as nhaH. The deduced amino-acid sequence of NhaH consists of 403 residues with a calculated molecular mass of 43 481 Da, which was 54% identical and 76% similar to the NhaG Na+/H+ antiporter of Bacillus subtilis. The hydropathy profile was characteristic of a membrane protein with 12 putative transmembrane domains. Everted membrane vesicles prepared from E. coli cells carrying nhaH exhibited Na+/H+ as well as Li+/H+ antiporter activity, which was pH-dependent with highest activities at pH 8.5–9.0 and at pH 8.5, respectively. Moreover, nhaH confers upon E. coli KNabc cells the ability to grow under alkaline conditions.