Editor: J. C. Murrell
Transcript profiles of Nitrosomonas europaea during growth and upon deprivation of ammonia and carbonate
Article first published online: 15 FEB 2006
FEMS Microbiology Letters
Volume 257, Issue 1, pages 76–83, April 2006
How to Cite
Wei, X., Yan, T., Hommes, N. G., Liu, X., Wu, L., McAlvin, C., Klotz, M. G., Sayavedra-Soto, L. A., Zhou, J. and Arp, D. J. (2006), Transcript profiles of Nitrosomonas europaea during growth and upon deprivation of ammonia and carbonate. FEMS Microbiology Letters, 257: 76–83. doi: 10.1111/j.1574-6968.2006.00152.x
- Issue published online: 15 FEB 2006
- Article first published online: 15 FEB 2006
- Received 1 December 2005; revised 9 January 2006; accepted 11 January 2006.
- Nitrosomonas europaea;
- transcript profiles;
- gene expression;
- ammonia deprivation;
The transcriptome of Nitrosomonas europaea was analyzed with whole-genome microarrays. Growing cells were compared to cells deprived of (NH4)2SO4 and Na2CO3. Hybridization signals were detected for 76% of the genes represented on the array under either or both conditions. Transcript levels for 68% of the genes were at least twofold higher in growing cells than in deprived cells, while only 0.42% of the genes were present at more than twofold higher levels in deprived cells. Transcript levels for the remaining 7% of the genes did not change significantly with the treatments. These trends were confirmed for selected genes by Northern hybridizations and quantitative RT-PCR. Compared to heterotrophic bacteria, N. europaea downregulates a greater proportion of its genes and fewer genes appear to be associated with the adaptation to starvation.