Editor: Jörg Simon
Ladderane phospholipids in anammox bacteria comprise phosphocholine and phosphoethanolamine headgroups
Article first published online: 21 APR 2006
FEMS Microbiology Letters
Volume 258, Issue 2, pages 297–304, May 2006
How to Cite
Boumann, H. A., Hopmans, E. C., Van De Leemput, I., Op den Camp, H. J. M., Van De Vossenberg, J., Strous, M., Jetten, M. S. M., Sinninghe Damsté, J. S. and Schouten, S. (2006), Ladderane phospholipids in anammox bacteria comprise phosphocholine and phosphoethanolamine headgroups. FEMS Microbiology Letters, 258: 297–304. doi: 10.1111/j.1574-6968.2006.00233.x
- Issue published online: 21 APR 2006
- Article first published online: 21 APR 2006
- Received 17 January 2006; revised 10 March 2006; accepted 13 March 2006.First published online April 2006.
- anammox bacteria;
- ladderane lipids;
- dense membrane;
Anammox bacteria present in wastewater treatment systems and marine environments are capable of anaerobically oxidizing ammonium to dinitrogen gas. This anammox metabolism takes place in the anammoxosome which membrane is composed of lipids with peculiar staircase-like ‘ladderane’ hydrocarbon chains that comprise three or four linearly concatenated cyclobutane structures. Here, we applied high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to elucidate the full identity of these ladderane lipids. This revealed a wide variety of ladderane lipid species with either a phosphocholine or phosphoethanolamine polar headgroup attached to the glycerol backbone. In addition, in silico analysis of genome data gained insight into the machinery for the biosynthesis of the phosphocholine and phosphoethanolamine phospholipids in anammox bacteria.